Suz12 binds to silenced regions of the genome in a cell-type-specific manner

Different PRC complexes regulate different types of Suz12 targets. Shown are the categories, as determined using the program DAVID, for (A) F9 cell Suz12⁺, Eed1⁺ and (B) F9 cell Suz12⁺, Eed1⁻ target genes. The black bars indicate Suz12 target genes that are stem cell or germ cell tumor specific, and the hatched bars indicate Suz12 target genes that are common to all cell types. Shown in parentheses are the P-values, which indicate the probability that the category has been identified by random chance. For the Suz⁺, Eed1⁺ targets, the median Suz12 value was 1.816 (log₂), and the median Eed1 value was 0.762 (log₂). For the Suz12⁺, Eed1⁻ targets, the median Suz12 value was 0.91 (log₂), and the median Eed1 value was 0.205 (log₂). (C) PCR confirmations were performed to confirm the existence of two classes of target genes, those bound by Suz12, Ezh2, and Eed1 and those bound by Suz12 and Ezh2, but not by Eed1.