Evidence for purifying selection on AmnSINE1 in mammals. (A) An example of conserved AmnSINE1 locus (the window is chr15:59,354,815-59,356,314 in which the AmnSINE1 position is located at chr15:59,355,417-59,355,712) obtained from UCSC Genome Bioinformatics Web site. Note that the AmnSINE1 sequence is conserved in all mammals including opossum. (B) The location and conservation of 10 representative AmnSINE1 loci in human. The position information in human was determined from genomic sequence data in UCSC Genome Bioinformatics (ver. hg17). (Chr) Chromosome number. PhastCons conservation scores of the 1.5-kbp region around and including each AmnSINE1 were obtained by comparing human, chimpanzee, mouse, rat, and dog sequences, and the graphs are displayed for each locus. In each graph, the black region denotes the AmnSINE1 sequence and the gray represents the flanking region corresponding to the given position. Detailed information for the 10 loci is available in Supplemental Figure 3.
