Figure 3.
Two P1 ATPases are necessary for resistance to four transition metals: Co(II), Ni(II), Cu(II), and Zn(II). Growth phenotypes of P1 ATPase gene knockout strains ΔyvgX and ΔzntA were determined as final cell density (OD600) after 27 h incubation at 37°C in increasing concentrations of (i)Co(II), (ii) Ni(II), (iii) Cu(II), and (iv) Zn(II). Results from growth rate assays on these strains in different metals are presented in Supplemental Figure 3. Δura3 is the parent strain in which gene deletions are constructed.
