
BAC length constraints derived from physical maps. (A) FASSI-1 consists of four different sets of length constraints between any two overlapping BAC neighbors (40% overlap in our implementation). In addition to introducing accurate length constraints between mate pairs of a given BAC, two additional, wide overlap constraints are introduced between neighbors. (B) Constraints in FASSI-6 include the length constraints from FASSI-1, plus short overlap constraints between any two proximal BAC end sequences from BAC sharing 40% of their length. The FASSI parameters are detailed in Methods.











