In situ detection of 8-oxoG on human metaphase chromosomes. (A) Immunological detection of 8-oxoG. The arrowhead indicates one example of a twin dot on sister chromatids. (B) Immunological detection of 8-oxoG after treatment with MutM. (C) MutM-dependent in situ end-labeling. (D) MutM (−) negative control for in situ end-labeling. (E) Signal patterns on chromosome 7: comparison between immunological detection (top) and MutM-sensitive sites (bottom). Lines indicate centromere. The monochrome images of signal (left) and DAPI (middle) are digitally enhanced and positively inverted. For a merged image, signal and DAPI images are pseudo-colored with green and red, respectively (right). (F) Signal positions on chromosome 7 are indicated as filled circles on corresponding regions in the ideogram of the G-banding pattern at the 850-band resolution obtained from the NCBI ftp site. The line indicates centromeres.
