Figure 6.
Amplification PCRs of a 500-bp synthetic chunk of yeast retrotransposon Ty1. The oligos used to assemble this single 500-bp chunk were optimized for a melting temperature of 56°C and then assembled with gradient PCR with annealing temperatures ranging from 45°C–65°C. Each assembly was then amplified at the same annealing temperatures. This gel shows the amplification results on the 20°C gradient with the lowest temperature on the right.
