Figure 5.
Oligo design and A+T content. To test the robustness of GeneDesign’s oligo design algorithm, 100 random 500-bp sequences with A+T content varying from 0% to 100% were generated by a Perl script and run through the oligo design module. The resulting chunks were analyzed for the length and melting temperature of their constituent oligos. The number of times consecutive oligos on the same strand overlapped (collision) or met without a gap (touch) was counted by a Perl script.
