Characterization of the deletion in the gibbon genome. (A) The putative deletion in the gibbon genome is projected on human 20q11. (Horizontal lines) Positions of the PCR amplicons; (black and gray lines) amplified and non-amplified amplicons, respectively. The positions of the human genomic contigs are also shown. (B) Positions of five PCR amplicons used to localize the break points. Estimated length of PCR amplicons are shown in parentheses. (Arrows) Primers (see Supplemental Table 3), (horizontal bars) human genomic contigs AL035458.35 and AL356299.16. For the gibbon genome with the putative deletion, amplicons a, b, and c are expected to give rise to specific amplification. By contrast, specific amplification of amplicons d and e is expected for the primates other than the gibbons. (C) The results of PCR amplification are illustrated. Letters above gel images correspond to PCR amplicons illustrated in panel B. Species of DNA template in each lane is shown below the images. (m) Molecular markers. Conditions of these PCR assays are summarized in Supplemental Table 4.
