Genomic organization around ASIP. Positions of ASIP and surrounding genes in mouse chromosome 2 (left) and human chromosome 20 (right) are illustrated (Deloukas et al. 2001; Waterston et al. 2002). cen and ter indicate directions for centromere and telomere, respectively. The characteristics of the array of Alu elements in 32.165–32.330 Mb of human chromosome 20 are also described (dashed box). Old Alu includes AluSx, AluSq, AluSg, AluSc, AluSp, AluS, AluJo, and AluJb subfamilies, while Young Alu includes AluY and AluYd2 subfamilies (Shen et al. 1991; Kapitonov and Jurka 1996; Batzer and Deininger 2002). Chemiluminescence images of genomic Southern blotting for RALY and ASIP are also shown. DNA samples loaded in each lane are as follows: (Human and Great Apes) H. sapiens, P. troglodytes, and P. pygmaeus; (Gibbons) H. lar (two individuals), H. pileatus, H. agilis, S. syndactylus, and N. leucogenys; (Old and New World Monkeys) M. fuscata, C. aethiops, and S. oedipus. Restriction enzymes used in the fragmentation of genomic DNA are also indicated (see Methods).
