Figure 2.
Sequence conservation of Ey binding sites in the so10 eye enhancer. (A) Sequence alignment of three Ey binding sites from eight Drosophila species. Positions 5, 9, 11, 13, and 14 (highlighted in boxes) are almost identical across all Drosophila species. (B) The human PAX6 position weight matrix (PWM) is represented by a logo, where nucleotide preference is represented by the height of the letter. This was combined with the so binding site data to build an Ey PWM, shown by the logo in C. (D) Optimized Ey PWM based on new Ey binding sites identified in this report. (Dm) D. melanogaster, (Dsi) D. simulans, (Dt) D. teisseri, (Dy) D. yakuba, (De) D. erecta, (Da) D. ananassae, (Dse) D. sechelia, (Dp) D. pseudoobscura.
