A two-member dCNE family (#464) located within the introns of FOXP1 (464_1) and FOXP2 (464_2). Multiple alignment of sequences was carried out using CLUSTALW (v1.83) (Thompson et al. 1994). Element boundaries were defined by sequence conservation between human and Fugu for each family member. Human–Fugu orthologs of 464_1 are conserved at 92.7% identity over 316 bases while orthologs of 464_2 are conserved at 88.4% identity over 199 bases between these species. Conservation between human copies of 464_1 and 464_2 across the length of the smaller element (248 bp) was 83.5%, lower than that seen between orthologous copies but considerably higher than the average conservation between human dCNEs (Fig. 5). In addition, these elements have a length ratio (see Methods) of 0.78 indicating significant evolution of the elements at their edges. 464_1 was not detected in chicken and 464_2 was not detected in chimp, possibly because of missing sequence in these assemblies.
