Figure 4.
Igf1r is mutated in the ENU-induced mutant hydrops fetalis (hyft). (A) Normal littermate (left) and hyft mutant (right) at E16.5, showing reduced fetal crown-to-rump length. Arrow points to subcutaneous edema present in the affected. (B) Western blot analysis of Igf1r on protein extracts of hyft mutants and normal littermates shows an absence of Igf1r protein in affected mice. (C) Chromatograms of Igf1r sequence in wild-type FVB (top) and hyft affected (bottom) mice showing a T to A mutation, which encodes a premature stop codon in hyft mutants.
