Decoding the fine-scale structure of a breast cancer genome and transcriptome

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Figure 2.
Figure 2.

Using tumor-derived BAC clones for phenotype screens. (A) Confirmation of retrofitting BAC MCF-7_1-3F5 for transfection studies. Arrows mark new bands in the vector resulting from insertion of pRetroES plasmid. (B) PCR-based control for transfection of EPH4 cells with retrofitted BAC clone. (Lane 1) Nontransfected cells; (lane 2) transfected cells; (lane 3) positive control (BAC DNA); (lane 4) negative control. (C) Increase in resistance of EPH4 cells to doxorubicin. Note the decrease in the number of apoptotic cells as demonstrated by the annexin assay.

This Article

  1. Published in Advance February 3, 2006, doi: 10.1101/gr.4247306 Genome Res. 16: 394-404

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