James P. Kastenmayer; Li Ni; Angela Chu; Lauren E. Kitchen; Wei-Chun Au; Hui Yang; Carole D. Carter; David Wheeler; Ronald W. Davis; Jef D. Boeke; Michael A. Snyder; Munira A. Basrai

Functional genomics of genes with small open reading frames (sORFs) in S. cerevisiae

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Figure 6.

Protein expression analysis of HA-tagged sORFs. Western blot analysis of protein extracts prepared from strains expressing HA-tagged ORFs (YJL062W-A, YPL189C-A, YGR271C-A/YGR272C, YDR524W-C) and the wild-type strain (BY4741) not expressing a HA-tagged protein (no HA tag). Proteins of the expected size were detected (ORF-HA), and Tub2p served as a loading control (Tub2).