Figure 4.
sORFs required for growth on media containing replication-arrest and DNA-damaging agents. Growth assays of 3 μL of fivefold serial dilutions of logarithmic-phase cells of the sORF deletion strains spotted on YPD plates (control) or spotted on YPD plates containing (A) 200 mM HU, (B) 10 mU/mL bleomycin, or (C) 0.02% MMS. For sensitivity to UV, we irradiated strains spotted on YPD with (D) 20 mJ/m2 of UV radiation. All plates were incubated at 30°C for 2 to 3 d. The checkpoint mutant (mec1Δ sml1Δ, U953–61D) and the wild-type strain (BY4741) served as controls.
