Figure 3.
sORFs required for growth at 37°C (Ts) or in the presence of nonfermentable carbon source (petite phenotype). (A) sORFs required for growth at 37°C. Growth assays of 3 μL of fivefold serial dilutions of logarithmic-phase cells of the sORF deletion strains spotted on YPD plates and incubated at 30°C or 37°C for 2 to 3 d. The wild-type strain BY4741 and the temperature-sensitive strain ndc10-1 (JK421) served as controls. (B) Growth assays of 3 μL of fivefold serial dilutions of logarithmic-phase cells of the sORF deletion strains spotted on YP Dextrose or YP Glycerol plates and incubated at 30°C for 2 to 3 d.
