Evidence of S. cerevisiae sORFs. (A) Gene expression-based analyses and homology searching reveal 170 potential sORFs, bringing the total number of annotated sORFs in S. cerevisiae to 299. Reports in the literature provided empirical evidence of transcription derived from SAGE, microarray, RT-PCR, Northern blot, and gene-trap experiments, while empirical evidence of translation was derived from reports of mass-spectrometry and gene-trap experiments. Comparative genomic studies provided evidence of homology. (B) The bar graph depicts the subcategorization of the evidence for the 170 new sORFs, showing that the largest number of sORFs were identified based on evidence of transcription followed by evidence based on translation and homology.
