
Chromosomal localization of sequenced BACs. FISH analysis of pachytene stage C. rubella (A,B,C) and O. pumila (D–L) chromosomes; each consists of synapsed homologs. Chromosomes (DAPI-stained, blue) were probed with centromere satellites (green) and BAC DNA (red). BAC probes include (A,B,C) Cr8F1; (D,E,F) Op44L14; (G,H,I) Op14G23; and (J,K,L) Op37I22. Merged images (center and right columns) show BAC locations (arrows) relative to DAPI-stained heterochromatin and centromere satellite arrays; similar staining patterns were observed on 10–50 pachytene chromosomes/probe.











