Figure 4.
Protein sequence alignment translated from the nucleotide alignment of the AmelFibroin proteins. The bulk of the nucleotide alignment (Supplemental Fig. II) was derived from aligning primary sequence and conserving heptad periodicity as described in the Methods. The alanine residues populating the hydrophobic heptad positions are marked (position a shaded and position d in bold). The predicted signal peptide is underlined. Regions shown in italic were aligned using MUSCLE.
