RDR2-independent small RNAs from regions with ta-siRNA-like features. (A) The locus that includes small49 exhibits 21-nt phasing and accumulation characteristics in mutants similar to those of ta-siRNAs. Image of the MPSS Web viewer for the intergenic region that contains small49 in the position indicated by the large gray arrow (the shaded square covering both strands in this intergenic region indicates an inverted repeat); an RNA gel blot of small49; a plot of the y-axis indicating the small RNA abundance in the rdr2 mutant as measured by MPSS (in TPQ), and the x-axis indicating nucleotide position on Chr. 1, with the “697” indicating position 25,282,697. (B) The blot shows that small58 also has ta-siRNA-like accumulation features; images as described in A, with the 0 position in the x-axis of the plot indicating nucleotide 13,295,900 on Chr. 4. Due to limited space, only a portion of this intergenic region is shown, with the flanking genes indicated at the ends by their At identifiers.
