Small RNAs or clusters common to wild type and rdr2. Venn diagrams representing genome-matched rdr2 454 and MPSS sequences from Table 1. (A) A comparison of distinct signatures in the MPSS libraries indicates 19% of rdr2 sequences were also found in wild type. (B) A comparison of distinct signatures in the 454 libraries indicates 21% of rdr2 sequences were also found in wild type. (C) A comparison of genomic clusters of MPSS signatures indicates 93% of small RNA clusters represented in rdr2 were also found in wild type. For this analysis, clusters contained at least three small RNAs across both libraries; this cutoff was chosen arbitrarily to remove clusters with only one or two small RNAs that could be background. Most of the rdr2-only clusters are low-abundance miRNAs or other “real” sequences that were not detected due to depth of coverage in the wild-type library.
