Figure 2.
An example of Southern blots used to measure genome content of telomere elements. Samples of DNA from the Oregon R and 2057 stocks probed with HeT-A ORF (lanes on left) and probed with TART ORF (lanes on right) were cut with BamHI (lane B), EcoRI (lane E), HindIII (lane H), or XhoI (lane X). Filters were first probed with sequence for rp49 as a loading control, and the relevant region of each filter is shown below the HeT-A/TART blots. Comparison of the restriction fragments shows that the two stocks differ significantly in the number and linear arrangement of different sequence variants of the telomere elements.
