Discovery, validation, and genetic dissection of transcription factor binding sites by comparative and functional genomics

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 4.
Figure 4.

Gel shift assays. (A) Gel shift showing the Cy3 labeled probe incubated with whole-cell extracts from stp2Δ cells, wild-type cells, or cells engineered to overexpress HA-tagged STP2. The black arrow represents the reproducible shift involving Stp2. Faster running bands represent possible cleavage or degradation products of Stp2. The last lane shows a super-shift with anti-HA antibody. (B) Gel shift showing STP2 overexpressing whole-cell extract with varying amounts of unlabeled wild type and mutant competitor.

This Article

  1. Genome Res. 15: 1145-1152

Preprint Server



Current Issue

  1. February 2026, 36 (2)
  1. Current Issue
  1. Alert me to new issues of Genome Research