Discovery, validation, and genetic dissection of transcription factor binding sites by comparative and functional genomics

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Figure 3.
Figure 3.

One-hybrid assay. (A) Ninety-six AD fusions mated to HIS3 reporter plasmid grown on 75 mM 3-amino triazole (3AT), a competitive inhibitor of the HIS3 gene product. The arrow points to the Stp2-AD fusion. (B) AD fusions mated to mutant versions of a HIS3 reporter plasmid. (C) Sequences used as promoters in one-hybrid assay. Red indicates the motif, and blue indicates mutations.

This Article

  1. Genome Res. 15: 1145-1152

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