Figure 1.
Chr 5Rw Inversion screen. Male C57BL/6J mice (generation G0) were treated with ENU to induce point mutations (*) and mated to C3H-Rw/+ females (the Rw inversion is designated as a thick, striped bar). G1 offspring inheriting Rw (white spotting on hindquarters) were selected and crossed to mice carrying Rw in trans to Hm, a semidominant mutation causing webbed digits. This permits visual selection of G2 progeny that are Rw/+* (they have white spotting, but no webbed digits). G2 animals were intercrossed to produce the three types of offspring shown. Rw/Rw embryos die in utero. Non-Rw G3 animals are homozygous for the ENU-exposed Chr 5, and were screened for phenotypes.
