Description of large constrained regions in the CFTR locus. (A) Sizes of constrained elements identified with a merging tolerance of six unconstrained columns, with the length in base pairs of each bin along the x-axis and the count for each bin along the y-axis. Bins are divided according to those elements that overlap exons (black) and those that do not (gray). (B) Large, non-coding constrained elements that overlap coding exons (see Methods). Each coding exon in the region is displayed in ascending order along the x-axis according to human genome coordinates. Exons are boxed according to which gene they belong, and transcription orientation of each gene is shown with an arrow. Note that in this format, the left-most exon is the first coding exon for all of those genes transcribed to the right, while the opposite is true for genes transcribed toward the left. The distance that the associated noncoding constrained elements extend away from the individual exons is plotted along the y-axis. Positive values are indicated for the 3′ direction, and negative values for the 5′ direction.
