Real-time PCR data on NF-κB association to one known and three predicted binding sites. The real-time PCR data correspond to the in vivo NF-κB association to known and predicted sites in the promoters of genes iNOS and autotaxin (two sites in each promoter). This graph confirms the direct association of NF-κB with iNOS114 (known site at position -114 from the TSS) and reveals that NF-κB binds on both predicted sites in autotaxin gene (at positions -375 and -596, respectively). The peaks show the maximum fold association compared to non-induced cells (control sample) in the time interval between 30-min and 2-h post-induction. This analysis does not confirm a second predicted iNOS site (at position -2760). All predictions were made by Footer, using the default parameters described in the text.
