Table 4.
A summary of pathogenicity-related genes and mutants identified in this studya
|
Functional category |
No. genes annotated in the genome |
No. genes identified from mutants |
Ratio of genes identified vs. annotated |
|---|---|---|---|
| Cell mobility | |||
| Type IV pili assembly | 26 | 2 | 0.077 |
| Flagellar assembly | 33 | 0 | 0 |
| Surface polysaccharides biosynthesis | |||
| Exopolysaccharides | 16 | 3 | 0.188 |
| Lipopolysaccharides | 57 | 10 | 0.158 |
| Cell-wall degradation enzymes and secretion system (SS) | |||
| Type II SS (xps system) | 11 | 6 | 0.545 |
| Type II SS (xcs system) | 12 | 0 | 0 |
| Cellulase | 9 | 1 | 0.111 |
| Other extracellular enzymes | 15 | 0 | 0 |
| Effectors and secretion system | |||
| Type III SS | 26 | 5 | 0.192 |
| Effectors | 8 | 0 | 0 |
| Other secretion systems | |||
| Type IV SS | 17 | 1 | 0.059 |
| Autotransporters | 5 | 0 | 0 |
| Detoxification and adaptation | |||
| Glutathione S-transferase | 9 | 1 | 0.111 |
| Catalase | 4 | 2 | 0.500 |
| Glutathione peroxidase | 4 | 0 | 0 |
| Fatty acid degradation | 13 | 2 | 0.154 |
| Chemotaxis | 46 | 1 | 0.022 |
| Related general metabolisms | |||
| TonB-dependent receptor | 79 | 4b | 0.051 |
| Amino acid biosynthesis | 115 | 10 | 0.087 |
| Purine biosynthesis | 22 | 3b | 0.136 |
| DNA restriction | 18 | 1 | 0.056 |
| Energy metabolism | 210 | 2b | 0.014 |
| Regulatory element | ND | 5 | ND |
| Othersc | ND | 16 | ND |
| Total
|
|
75
|
|
-
↵a Details of genes identified from virulence-reduced mutants are listed in Supplemental Table 3.
-
↵b Signifies inclusion of intergenic mutations that might affect the transcription of functional genes.
-
↵c This category contains function-unknown genes (including Xcc 8004 strain-specific genes) and part of the intergenic mutations.
