Table 1.
Tilling results: Overview
|
|
125 mM EMS |
50 mM EMS |
Total |
|---|---|---|---|
| G0 mutagenized # | 10000 | 10000 | |
| G0 survivers # | ∼2500 (25%) | ∼9000 (90%) | |
| F1 #a | 700 | 3920 | |
| Number of linesb | 171 | 1915 | 2086 |
| F2 viable chromosomes (f)c | 9/171 (5.2%) | 41/384 (10.6%) | |
| Lethal hits per chrom. (λ)d | 2.94 | 2.24 | |
| Amplicons screenede | Sara-1, 2, 3, 4, 5 | Sara-1, 2, 3 (187) | |
| Alp23B-1, 2, 3 | Sara-4, 5 (1915) | ||
| Arf6-1, 2 | Alp23B-1, 3 (187) | ||
| Alp23B-2 (1915) | |||
| Arf6-1, 2 (187) | |||
| Candidates after tillingf (LI-COR hits) | 1 signal: 1 | 1 signal: 73 | |
| 2 signals: 13 | 2 signals: 33 | ||
| Total 14 | Total 106 | 120 | |
| Confirmed by sequencingf | 1 signal: 0 | 1 signal: 15 (20.5%) | |
| 2 signals: 12 (92.3%) | 2 signals: 17 (51.5%) | ||
| Total 12 | Total 32 | 44 | |
| Total screenedf | 1086.534 kb | 4977.702 kb | 6064.236 kb |
| Frequencyf | 1 in 90.5 kb | 1 in 155.6 kb | — |
| Missense SNPsf | 10 (83.3%) | 23 (71.9%) | 33 (75.0%) |
| Splice acceptorf | 0 | 1 (3.1%) | 1 (2.2%) |
| Silentf | 2 (16.7%) | 8 (25%) | 10 (22.7%) |
| C/G to T/A transitionf | 9 (75%) | 28 (87.5%) | 37 (84.1%) |
| Other mutationsf | 1 T/A to C/G | 1 T/A to G/C | 5 T/A to A/T |
| 2 T/A to A/T | 3 T/A to A/T | 1 T/A to C/G | |
|
|
|
|
1 T/A to G/C
|
-
↵a Total number of b p cn sca*/CyO, hb-lacZ F1 males.
-
↵b Total number of F2 crosses that produced progeny.
-
↵c Total number of lines where homozygous b p cn sca* adults survive. Denominator, total number of lines inspected.
-
↵d Lethal hits per chromosome: λ = -Inf.
-
↵e 171 lines were screened for each amplicon in the case of the 125 mM mutagenesis. For the 50 mM mutagenesis, the number of lines screened for a particular amplicon is indicated in parenthesis.
-
↵f Repeated signals due to overlap of the amplicons are only represented once.
