Figure 2.
A flow chart of the IVV analysis system for the sequence data. The automatic sequence data analysis system provides a positive data set for PPI analysis (Fig. 5) as described in “Identification of positives for PPI mapping and in silico analysis” in the protocol section. The manual process (broken arrows) corresponds to post-selection, which consists of in vitro experiments after selection to identify independently final positives and further false positives using a real-time PCR assay (Fig. 3A) and a pull-down assay (Fig. 3B).
