CADLIVE dynamic simulator: Direct link of biochemical networks to dynamic models

The GS concentrations of a wild-type and knockout mutants were simulated in an ammonia-limiting or ammonia-rich medium to compare with those of experimental data.

(A) The GS concentrations were compared between an ammonia-rich medium and an ammonia-limiting medium, where a wild type greatly increased the GS concentration due to the Ntr response. When the cells were transferred from an ammonia-rich medium to an ammonia-limiting medium, the GS concentration increased more than five-fold (++), increased five- to two-fold (+), and hardly changed (less than two-fold) (±). (B) The GS concentrations of the mutants were compared with those of a wild type in an ammonia-rich medium. Compared with the wild type, the GS concentration of mutants increased five-fold (++), increased five- to two-fold (+), hardly changed (±), and decreased (–).