Analysis of H2 haplotype conservation in the wheat gene pool. (A.) Schematic representation of the amplified fragments spanning the RGA2 fragments of T. aestivum. (B.) PCR amplification of 331-bp fragment (B) and the positive control for amplification (+). PCR fragments span the 3′ end of RGA2 for the amplification control and the deletion breakpoint for the B fragment. Two T. monococcum lines (Tm, lanes 1,2, TRI 2006 and TRI 17434), one T. urartu line (Tu, lane 3, TRI 17413), three T. turgidum subsp. dicoccoides lines (Tdi, lanes 4–6, TRI 3425, TTD22, IG 46516), one T. turgidum subsp. durum line (Tdu, lane 7, TRI 673), one T. timopheevii subsp. armeniacum line (Tti, lane 8, TA 952) and three T. aestivum lines (Tae, lanes 9–11, cvs. Chinese spring, Arina, and Renan) of the haplotype H2 are shown. (L) 1 kb ladder; (C) water control.
