Whole genome shotgun sequencing of Brassica oleracea and its application to gene discovery and annotation in Arabidopsis

Table 3.

Success rate in validating expression from singleton and paired CAGS



No. of CAGS

Fraction of CAGS expressed
CAGS category
Tested
With high quality sequences
Experimental sequence matching target
Total
Truly intergenica
Perigenica
Singleton 109 69 20 18.3% (20/109) 20.6% (15/73) 13.9% (5/36)
Pair
83
64
38
45.8% (38/83)
47.3% (26/55)
42.9% (12/28)
  • a The original set of intergenic CAGS was further divided into those close to but not intersecting existing gene annotations (perigenic) and those sufficiently removed from existing annotations to be likely novel conserved structures (see text for details)

This Article

  1. Genome Res. 15: 487-495

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