Table 6.
Verification of the expression data retrieved from the microarray comparison of flowers versus leaves with real time reverse transcriptase PCR.
|
|
|
|
Quantitative RT-PCR |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Microarray |
Raw (Ct) |
Normalized ΔCt |
|||||||||
| EST ID |
Annotation |
Average ratio F/L |
Flower |
Leaf |
Flower |
Leaf |
Ratio F/L |
||||
| G0000100033H3 | Polyphenol oxidase | 0.41 | 18.44 | 25.99 | 3.61 | 11.51 | 0.004 | ||||
| G0000100002G1 | Flavonoid 1-2 rhamnosyltransferase | 0.73 | 24.38 | 26.34 | 9.55 | 11.86 | 0.202 | ||||
| G0000300001A5 | Non-specific lipid transfer protein | 1.49 | 22.08 | 19.61 | 7.25 | 5.13 | 4.347 | ||||
| G0000300017G4 | Cytochrome P450 | 0.32 | 23.07 | 25.66 | 8.24 | 11.18 | 0.130 | ||||
| G0000700010E10
|
T-complex protein
|
0.75
|
21.12
|
20.99
|
6.29
|
6.51
|
0.859
|
||||
-
The raw threshold cycle (Ct) values were normalized against a ubiquitin standard to get normalized ΔCt values. The expression differences in the leaf and flower sample were calculated from the formula 2ΔCt flower / ΔCt leaf. F = flower, L = leaf.
