Post-entrapment genome engineering: First exon size does not affect the expression of fusion transcripts generated by gene entrapment

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Figure 2.
Figure 2.

Replaceable 3′ gene entrapment cassettes. (A,B) neomycin- and (C,D) zeocin-resistance genes ending at a 3′-splice site (SD) and RNA destabilization sequence (flash symbol) were cloned downstream of the (A) RNA polymerase II (Pol II) or (B,C,D) phosphoglycerol kinase (PGK) promoters. IntZeoPTC contains a synthetic intron inserted within the Zeocin-resistance gene (D). After gene entrapment, vector sequences splice to downstream exons of cellular genes (black boxes). The sizes of 5′-exons containing Neo and Zeo sequences is indicated in base pairs (bp). Heterospecific loxP sites (loxP and lox5171) allow vector sequences to be replaced by Cre-mediated cassette exchange.

This Article

  1. Genome Res. 15: 428-435

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