Protein interaction mapping: A Drosophila case study

Table 1.

General features of the Drosophila protein interaction map




Value

Comments
Screens 154
Combinations of bait/prey polypeptide tested 7.7 × 109
Preys tested/bait 5 × 107
Selected colonies/bait 0-380 On average 119 colonies/bait
Selected colonies/million pairs tested 1.7
Selected clones 18,353
Sequenced clones 16,123 88% of selected clones
Clones identified 15,201 94.3% of sequenced clones
6.7% not predicted cDNA
Protein-protein interactions 710 (PBS A to C)
2185 (PBS A to D)
2338 (PBS A to E)
Proteins connected 641 (PBS A to C)
1711 (PBS A to D)
1727 (PBS A to E)
Mean connectivity 2.22 (PBS A to C) 2.9/bait (PBS A)
2.55 (PBS A to D) 5.8/bait (PBS A, B)
2.71 (PBS A to E) 7/bait (PBS A to C)
21.4/bait (PBS A to D)
SID identified
2484

This Article

  1. Genome Res. 15: 376-384

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