Figure 2.
Validation of putative bimorphic SINE insertions. Six representative examples (a-f) of PCR products from loci that were predicted to contain bimorphic SINE insertions after analysis of SINEC_Cf libraries (see Fig. 1). Primers were designed to the flanks of the predicted insertion site, and templates were genomic DNA from a combination of mixed-breed dogs (lane 2), three Bernese Mountain dogs (lanes 3-5), three pugs (lanes 6-8), and three dachshunds (lanes 9-11). Product lengths were derived from DNA markers (lanes M), and were consistent with the presence (+) or absence (–) of a SINE insertion.
