Table 2.
Summary of microfabricated devices for DNA sequencing applications
|
Research group |
Number of channels |
Template source |
Separation length (cm) |
Accuracy (%) |
Read-length (bp) |
Read-out time (min) |
|---|---|---|---|---|---|---|
| Single channel | ||||||
| Woolley & Mathies (1995) | 1 | M13mp18 | 3.5 | 97 | 147 | 9 |
| Liu et al. (1999) | 1 | M13mp18 | 6.5 | 99.4 | 500 | 20 |
| Schmalzing et al. (1999) | 1 | M13 clonesa | 11.5 | 99 | 505 | 27 |
| Salas-Solano et al. (2000) | 1 | M13mp18 | 11.5 | 98.5 | 640 | 30 |
| Boone et al. (2002) | 1 | M13mp18 | 18.0 | 98 | 640 | 30 |
| Shi & Anderson (2003) | 1 | Unknown | 4.5 | 99.1 | 320 | 13 |
| Multiple channel arrays | ||||||
| Liu et al. (2000) | 16 | M13mp18 | 7.5 | 99 | 457 | 16 |
| Simpson et al. (2000) | 48 | M13mp18 | 10.0 | 97 | 400 | 25–45 |
| Backhouse et al. (2000) | 48 | BigDye Std | 46.5 | 98 | 640 | 150 |
| Koutny et al. (2000) | 32 | M13 clonesa | 40.0 | 98 | 800 | 78 |
| Paegel et al. (2002)
|
96
|
M13mp18
|
15.9
|
99
|
430
|
24
|
-
↵a Mixture of M13mp18 vector or twelve M13 clones from human chromosome 17 project
