Figure 4.
CRT technologies. (A) The CRT cycle. (B) The photocleavage reaction of a 3′-O-2-nitrobenzyl-nucleoside. (C) Effect of cycle efficiency on CRT read-length. (D) Kinetic study of protocleavage reaction for single substituted (2-SSNB) and double substituted (2-dsNB) 2-nitrobenzyl thymidine analogs. Percentage thymidine (%Thy) was calculated according to the equation: %Thy = AThy/(AThy + As2NB), where AThy and As2NB are the integrated peak areas from RP-HPLC analysis for thymidine and substituted 2-nitrobenzyl thymidine analogs, respectively.
