Figure 2.
Large-scale gain-of-function screen strategy in Xenopus. A unique full-length clone set is established and arrayed into 96-well plates. Miniplasmid preps are made and pools of plasmids made for each column. Each pooled set of plasmids is transcribed into mRNA in vitro. Pooled mRNA is injected into oocytes or embryos, and then a variety of functional screens are performed, depending on the type of molecules that are being sought. Once an active pool is identified, the pool is broken down to individual clones and assayed again to identify the active clone.
