Statistical parameters describing gene expression in single (insulin-expressing) β-cells at 5 and 20 mM glucose
|
Glucose |
Gene |
Na |
Arithmetic meanb |
Geometric meanc |
log10 Geometric mean (SD)d |
Shapiro-Wilk P-valuee |
Skewnessf |
|---|---|---|---|---|---|---|---|
| 5 mM | ActB | 51 | 130 | 61 | 1.79 (0.51) | 0.56 | -0.02 |
| Ins1 | 70 | 1700 | 190 | 2.29 (0.94) | 0.32 | 0.06 | |
| Ins2 | 44 | 5200 | 1100 | 3.03 (1.03) | 0.005 | -0.12 | |
| Abcc8 | 28 | 130 | 98 | 1.99 (0.34) | 0.97 | -0.05 | |
| Kcnj11 | 15 | 30 | 24 | 1.38 (0.22) | 0.77 | 0.31 | |
| 20 mM | ActB | 45 | 420 | 300 | 2.47 (0.37) | 0.53 | 0.01 |
| Ins1 | 49 | 7700 | 3200 | 3.51 (0.67) | 0.09 | -0.70 | |
| Ins2 | 47 | 16000 | 10000 | 4.01 (0.53) | 0.05 | -0.70 | |
| Abcc8 | 23 | 180 | 150 | 2.16 (0.33) | 0.21 | -0.01 | |
|
|
Kcnj11
|
18
|
34
|
31
|
1.49 (0.23)
|
0.48
|
-0.19
|
-
↵a N is the number of cells expressing the tested gene. A total of 60 (84 for Ins1 and Ins2) cells were collected in 5 mM glucose, and 61 (85 for Ins1 and Ins2) cells in 20 mM glucose
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↵b The arithmetic mean is calculated as μA = (Σ Xn)/N
-
↵c The geometric mean is derived from the formula μG = (Π Xn)1/N
-
↵d Logarithm of the geometric mean and corresponding standard deviations
-
↵e For the Shapiro-Wilk test, a high P value reflects a good fit. If P ≥ 0.05, then the null hypothesis that data are lognormal cannot be rejected
-
↵f Skewness is calculated as γ = Σ(Xn - μ)3/(N - 1)σ3
