Relative expression levels of Igf2r and Air. Quantitative real-time PCR analysis of Igf2r and Air expression. Total RNA from organs of four different adult male C57Bl6/CBA mice (sample C was obtained by pooling organs of two individuals) and 15.5-dpc embryos was reverse transcribed and analyzed for Air (shaded bars) and Igf2r (open bars) expression by qPCR using Taqman probes. Expression data were normalized to Cyclophilin A mRNA, and Igf2r and Air expression in the heart of sample B was set to 100 (marked by an asterisk). The numbers on the x-axis indicate the relative amounts of Igf2r and Air in different tissues but do not make a comparison between Igf2r and Air (see Supplemental Table 2 for the data used). The standard deviation representing three technical replicates for each sample is indicated. (E15.5 +/+) Wild-type embryo at 15.5 dpc. The E15.5 +/Thp embryo littermate that has a paternal deletion of the proximal Igf2r/Air imprinted cluster (Barlow et al. 1991) was used as a control for background levels of Air RNA.
