The relative intensities of markers under pyrimethamine selection, their positions on the P. c. chabaudi linkage map, and the positions of their homologs on P. falciparum chromosome 4, the location of pfdhfr
|
Marker namea |
Comparative marker intensityb |
Genetic distance (cM) from dhfr (P. c. chabaudi)c |
Physical distance (kb) from dhfr (P. falciparum)d |
|---|---|---|---|
| AJAA05CA | 0.00 | 5.3 | 10 |
| AJAG02CA | 0.13 | 9.5 | n/a |
| AJTG01AG | 0.06 | 13.4 | 35 |
| AJTG01GA | 0.08 | 13.4 | n/a |
| AJAC02TT | 0.42 | 33.0 | 119 |
| AJAA03AT | 0.60 | 44.5 | n/a |
| AJAA01GAe
|
1.02
|
53.5
|
n/a
|
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The comparative intensities of the markers are seen to decrease with increasing proximity to the gene under selection.
n/a = not available.
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↵a AFLP markers were named as follows: the first two letters identify the clone to which a marker is specific (AJ), the next two letters indicate the EcoRI primer selective bases (e.g., AA), the numbers identify the marker for that clone and primer combination in order of its molecular size, and the last two letters identify the Msel selective bases
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↵b Comparative Marker Intensity is a measure of the intensity of a marker in the selected population compared to its intensity in the unselected population
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↵c Markers were assigned to a linkage map between AS and AJ as described in Methods
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↵d P. c. chabaudi marker sequences were blasted against the P. falciparum genome to determine the positions of their homologs
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↵e This marker is not under pyrimethamine selection, and is included as an example of a flanking marker to give an indication of the size of the selection valley
