(A) Web site entry panel for query transcript At1g10200, with default precursor and miRNA parameter options. (B) Output from above query. (Graphic) Shows a map of the query transcript, below which is marked the positions of potential target sites (filled squares). These are labeled with the intergenic regions containing the precursor candidate loci from which the predicted miRNA candidates originate. The two labels on the right of this map, At1g20380 and At1g76140, correspond to the two cand2 loci that have Rfam names MIR394a and MIR394b, respectively. (Text) The predicted miRNA sequences are shown (white text on black), and, for comparison in this figure, the known mature miRNAs are indicated above the predicted precursors. When the `predicted orthologs in rice' box for the query options is also checked, only the At1g76140 cand2 candidate (MIR394b) record is returned. The At1g20380 cand2 candidate (MIR394a) record is not returned because the predicted miRNA for this record is 2 nt longer and does not have an exact match in rice. Boxes on the left can be checked and the precursor candidates aligned with ClustalW or downloaded in fasta format using the option shown below the listed candidate records.
