High-throughput functional screen of mouse gastrula cDNA libraries reveals new components of endoderm and mesoderm specification

Table 1A.

Clones identified by endoderm or mesoderm phenotypic effect on Xenopus embryos






Molecular marker


Gene
Group
Accession #
MGI #
Xsox17 β
edd
Xbra
# cloned
Library
Hspa8 un NM_031165 MGI:105384 + + 1 6.5
Kif22 mt NM_145588 MGI:109233 + + 1 7.5
Fzr1; Cdh1 cc NM_019757 MGI:1926790 + + 1 6.5
Mixl1 tf NM_013729 MGI:1351322 + + 2 6.5
Foxa2 tf NM_010446 MGI:1347476 + 1 7.5
Tcfeb tf NM_011549 MGI:103270 + 1 6.5
Otx2 tf NM_144841 MGI:97451 + 1 6.5
Habp2 ex NM_146101 MGI:1196378 + 1 7.5
Ccnf; cyclin F cc NM_007634 MGI:102551 1 6.5
Cks2 cc NM_025415 MGI:1913447 1 7.5
Rsk4 sg NM_025949 MGI:1914321 1 6.5
D7Ssb2 un AK002578 MGI:1914859 1 6.5
Gmnn; geminin cc NM_020567 MGI:1927344 2 7.5
Lefty2 ex NM_177099 MGI:2443573 5 6.5(4), 7.5(1)
Fgf8a ex NM_010205 MGI:99604 + 1 6.5
Fgf8f ex BC048734 na + 1 6.5
ActRIIb
tm
NM_007397
MGI:87912


+
1
6.5
  • Pluses indicate ectopic expression of the listed marker in whole embryos by in situ hydridization analysis. Minuses indicate loss of expression of listed marker in whole embryos by in situ hybridization. Table includes the gene name, functional group, accession number, mouse genomic resource number (MGI; see http://www.informatics.jax.org), number of times the clone was identified and the cDNA library that produced the clone. Functional group abbreviations are as follows: (cc) cell cycle component; (tf) transcription factor; (ex) extracellular secreted molecule; (sg) intracellular signaling molecule; (tm) transmembrane protein; (mt) microtubule assembly protein; (mb) metabolic protein; (un) unknown.

This Article

  1. Genome Res. 15: 44-53

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