Figure 4.
Positive clones that cause inhibition of mesoderm or endoderm in whole embryos. Gastrulating Xenopus embryos expressing positive clones were analyzed for the expression of Xbra (A,C,E,G,I,K), a combination of edd and Xsox17β (B,D,F) or edd alone (H,J,L). Identities of clones are listed at left and include Lefty2 (C,D), geminin (E,F), cyclin F (G,H), Cks2 (I,J), and D7Ssb2 (K,L). (A,B) Uninjected controls. Concentrations of injected mRNAs are as follows: Lefty2 (A,B: 1 ng), geminin (C,D: 400 pg), cyclin F (G,H: 200 pg), Cks2 (I,J: 200 pg), and D7Ssb2 (K: 10 ng; L: 500 pg).
