High-throughput functional screen of mouse gastrula cDNA libraries reveals new components of endoderm and mesoderm specification

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Figure 2.
Figure 2.

Positive pools were split as shown in this diagram. Ten subpools were generated of overlapping rows and columns. These subpools were injected and analyzed by in situ hybridization. Individual cDNA clones(s) within the intersection of phenotypically positive subpools were isolated and sequenced. This overlapping subpooling approach allows for the identification of positive clones in a single split.

This Article

  1. Genome Res. 15: 44-53

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