Transcriptome analysis for the chicken based on 19,626 finished cDNA sequences and 485,337 expressed sequence tags

Table 1.

Summary of chicken cDNA clone statistics


EC contigs selected for sequencing

22,607
   Coding 16,300
   Non-coding 6307
Clones attempteda 23,115
Clones accepted by EMBL database 19,626 (85%)
   Average length 1006 bp
   Number >1 Kb 7967
   Number >2 Kb 354
   Longest clone (ChEST76n19) 5339 bp
Clones annotated at 07/07/04 19,626
Accepted putative coding cDNAs 14,735
   That are spliced 10,901
Accepted putative noncoding cDNAs 4891
   That are spliced 1064
Clones mapped to genomeb 17,896
Clones mapped to genomec 18,519
Clones mapped to Ensembl transcript 12,211
Putative antisense clonesd 289
Putative noncoding RNAse 367
Conserved noncoding clonesf
43
  • a Multiple clones for some ECs were attempted when the most 5′ clone failed

  • b Ninety-eight percent identity over minimum of 100 bp via BLASTN

  • c Exonerate matches with a rawscore > 1000

  • d cDNAs found antisense to ensembl genes with at least five component ESTs in corresponding EST contig, and passing reverse clone quality control tests

  • e Clones mapped to assembled genome, but not found to be within 5 Kb of an Ensembl chicken gene

  • f Clones found in set e that which have a sequence that is also conserved in the human, mouse, and rat genomes

This Article

  1. Genome Res. 15: 174-183

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