Retrospective analysis of universal probe–target sequence mismatches and hybridization signal. Overgo-hybridization probes (n = 341) designed from human sequence and used to screen chimpanzee, baboon, cat, dog, cow, and pig BAC libraries (Thomas et al. 2002) were compared to newly available genomic sequence from each of these six target species. Local probe–target species sequence alignments were extracted from long-range human–target species genomic sequence alignments, and the number of mismatches recorded for each probe–target sequence comparison. The probe–target species sequence mismatch information was then combined with the probe hybridization results. A graph of the fraction of probe–target pairs that yielded a positive hybridization signal for a given number of probe–target sequence mismatches is indicated.
