Sequence organization of Galluhop and Charlie. (A) Organization of autonomous and nonautonomous Galluhop elements. The nonautonomous elements presumably originated from a deletion within the coding region of the transposase. Start and end positions of the coding sequence, as well as positions of the deleted region refer to consensus sequences for both the mother element and the deletion derivative. Both consensus sequences are available as Supplemental material (B) One Charlie subfamily carries an insertion of a nonautonomous Galluhop element. Positions of the Galluhop insertion refer to a Charlie/Galluhop fusion consensus sequence. (C) Detailed view of the Galluhop insertion site as shown by an alignment of the sequence Charlie12 (C12) from RepBase and the Charlie/Galluhop fusion consensus sequence (CG). The Galluhop element inserted at the position corresponding to position 790 in Charlie12 and has created a 2-bp putative target site duplication. The Charlie/Galluhop fusion consensus sequence (Charlie_Galluhop_fusion) is available as Supplemental material.
